I am interested in the mechanisms that control gene expresion and how extracellular signals can modulate the expression of the genes. I concentrated in stress signals that are transduced to the nucleus by Mitogen Activated Protein Kinases (MAPK). In yeast there are MAPKs homolgous to the human ones and the mechanisms of action are partially conserved. So yeast is an excellent organisms to study the molecular mechanisms by which MAP kinases control gene expression: how the MAP kinases modify and regulate transcription factors, how MAP kinases themselves interact with RNA pol II and with chromatin remodelling proteins, and how they control mRNA stability
Blondel, M., P. M. Alepuz, L. S. Huang, S. Shaham, G. Ammerer and M. Peter., 1999. Nuclear export of Far1p in response to pheromones requires the export receptor Msn5p/Ste21p. Genes & Development, 13 (17): 2284-2300.
Alepuz, P. M., Jovanovic, A., Reiser, V. and Ammerer, G., 2001. Stress induced MAP kinase Hog1 is part of transcription activation complexes. Mol. Cell, 7: 767-777.
Alepuz, P. M., de Nadal, E., Zapater, M., Ammerer, G. and Posas, F., 2003. Osmostress induced transcription by Hot1 depends on a Hog1-mediated recruitment of the RNA polymerase II. EMBO J., 22 (10): 2433-2442.
de Nadal, E., Zapater, M., Alepuz, P. M., Sumoy, L., Mas, G. and Posas, F., 2004. Regulation of gene expression by the Hog1 MAPK is mediated by the Rpd3 histone deacetylase. Nature, 427: 271-274.
PÃ¯Â¿Â½rez-OrtÃ¯Â¿Â½n, J. E., Alepuz, P. M. and Moreno, J., 2007. Genomics and gene transcription kinetics in yeast. TIG, in revision.